Main
Hédia Tnani
Education
Master in TICV
National School of Engineering (ENIT)
Tunis, Tunisia
2019 - 2020
- Graduate Program in System Biology, Machine Learning, Image Processing and Deep Learning
- Skills: Python programming, Image processing using OpenCV, Machine Learning algorithms, Time series
Master in Big Data
Centrale University (UC)
Tunis, Tunisia
2018 - 2019
- Graduate Program in Big Data and Machine Learning
- Skills: Big data tools (Hadoop, Spark, Storm, Flink, Flume, Sqoop), NoSQL databases (MongoDB, Cassandra, CouchDB), Object oriented programming
PhD in Biotechnology
Centre for Research in Agricultural Genomics (CRAG)
Barcelona, Spain
2007 - 2012
- Research project: The structure and function of maize scutellum during early stages of germination
- Scholarship: Becas Predoctoral I3P
- Advisor: Carlos Vicient
- The structure and function of maize scutellum during early stages of germination
- Construction of cDNA library for macroarrays and gene expression analysis of the microarrays
- Transgenic plant characterization and mutant plant analysis
- Confocal microscopy and subcellular localization
- Quantitative subproteomic analysis of germinating related changes in the scutellum oil bodies of Zea mays
- Molecular chracterization of ZmPTR1, a maize peptide transporter expressed in the epithelial cells of the scutellum during germination
- Bioinformatics tools: primer design, DNA/RNA sequence analysis, sequence alignment (GenBank,NCBI, TIGR Maize Database, Maize GDB, MSU Rice Genome Annotation Project)
- Agrobacterium-mediated transformation of Arabidopsis thaliana using the floral dip method
Master in Plant Breeding
CIHEAM Zaragoza, Mediterranean Agronomic Institute of Zaragoza
Zaragoza, Spain
2004 - 2007
- Graduate Program in Plant breeding
- Dissertation:The structure and function of maize scutellum during early stages of germination
- Advisor: Carlos Vicient
Research Projects
PostDoctoral Fellow
Pasteur Institute of Tunis (IPT). Laboratory of Bioinformatics, Biomathematics and Biostatistics (BIMS)
Tunis, Tunisia
2018 - Currently
- Drug discovery: Study of gene expression during infection to understand the Host-Pathogen interaction of L.major and Network Propagation analysis
- Identification of novel drug targets in Leishmania major using in-silico metabolic coupling
- Part of the H3ABioNet Workflows, Machine Learning, RefGraph, Gene Association WPs
- Development of Shell, R and Python pipelines for bioinformatics data analysis
- Cloud computing using AWS
- Nextflow pipeline for Variant Calling analysis and RNA-seq
- Continuous Integration of the bioinformatic workflows
PostDoctoral Fellow
International Rice Research Institute (IRRI)
Los Baños, Philippines
2013 - 2017
- Project manager of a BMZ project on developing flood tolerant rice varieties
- Fine mapping and candidate gene analysis of a QTL for anaerobic conditions during germination
- Identification of new QTLs for tolerance to anaerobic conditions using Genome Wide Association Studies (GWAS) and 3K Rice Genomes Project
- RNA-seq data analysis of anaerobic germination (AG) tolerant varieties
- SNP Data Analysis and QTL mapping: Plink, Haploview,TASSEL, FLAPJACK, GAPIT,GenomeBrowse, IGV
PostDoctoral Fellow
National Centre of Scientific Research (CNRS)
Rouen, France
2012 - 2013
- Project manager of the research aspect of an Interreg project between France (CNRS / University of Rouen) and England (University of Portsmouth)
- Development of bio-based cleaning wipes and filters using antiviral effects of polyphenols
- Protein analysis (plant protein extraction, viral protein extraction, SDS page analysis, 2D electrophoresis analysis)
Projects
Teaching Experience
Teaching Assistant (TA)
Higher School of Statistics and Information Analysis (ESSAI)
Tunis, Tunisia
2020
Grants
Predoctoral I3P Scholarship
N/A
Barcelona, Spain
2007 - 2012
- Superior Council of Scientific Research Predoctoral I3P fellowship (CSIC)
CIHEAM Scholarship
N/A
Zaragoza, Spain
2004 - 2007
- International Center for Advanced Mediterranean Agronomic Studies fellowship (CIHEAM)
Publications in Journals
Enriched-GWAS and Transcriptome Analysis to Refine and Characterize a Major QTL for Anaerobic Germination Tolerance in Rice
International Journal of Molecular Sciences
N/A
2021
- Tnani H, Chebotarov D, Thapa R, Ignacio JCI, Israel WK, Quilloy FA, Dixit S, Septiningsih EM, Kretzschmar T
ZmPTR1, a maize peptide transporter expressed in the epithelial cells of the scutellum during germination
N/A
Plant Science 2013, 207: 140-147
2013
- Tnani H, López I, García-Muniz N, Vicient CM
Expression profile of maize (Zea mays) scutellar epithelium during imbibition
N/A
Journal of Plant Physiology 2012, 169: 1430–1433
2012
- Tnani H, García-Muniz N, Vicient CM, López I
Control of the scutellar epithelial cell elongation during germination in maize (Zea mays L.)
N/A
Seed Science and Technology 2011, 39: 253-258
2011
- Tnani H, López I, Vicient CM
Protein composition analysis of oil bodies from maize embryos during germination
N/A
Journal of Plant Physiology 2011, 168: 510-513
2011
- Tnani H, López I, Jouenne T, Vicient CM
Workshops taught
RNA-Seq Downstream analysis using R
International Rice Research Institute (IRRI)
Los Baños, Philippines
2019
- Workshop on R for Genomic Data Analysis
Oral Communications
Differential quantitative proteomics analysis of the phage T4 under exposure to polyphenols using Label-free LC-MSMS
IRRI PBGB seminar
Los Baños, Philippines
2014
- Tnani H, Cosette P, Jouenne T
Gene expression in the scutellum of germinating maize
ISSS Workshop on Molecular Aspects of Seed Dormancy and Germination
Salamanca, Spain
2007
- Tnani H, López Ribera I, Vicient CM
Temporal and spatial variations of maize scutellum transcriptome during germination
IVth Workshop on Plant physiology. Catalan Society of Biology
Barcelona, Spain
2006
- Tnani H, López Ribera I, Vicient CM
Poster Presentations
Fine mapping and candidate gene analysis of a QTL for anaerobic conditions during germination
4th International Rice Congress
Bangkok, Thailand
2014
- Tnani H, Ignacio JC, Pelayo MA, Israel WK, Kretzschmar T, Septiningsih E
A new maize peptide transporter involved in the transport from the endosperm to the embryonic axis
Xth Congress Of Plant Molecular Biology
Valencia, Spain
2010
- Tnani H., López Ribera I., Vicient Sánchez CM
Gene expression in the scutellum of wild-type and dwarf1 maize mutant during germination
20th International Conference on Plant Growth Substances
Tarragona, Spain
2010
- Vicient Sánchez CM, Tnani H, López Ribera I
The transcriptome of maize scutellum
IXth Congress Of Plant Molecular Biology
Santiago de Compostela, Spain
2008
- López I, Tnani H, Miquel M, Vicient CM
Expression profile of maize scutellum
VIIIth Congress of Plant Molecular Biology
Pamplona, Spain
2006
- López I, Sánchez-Pons N, Miquel M, Bastida M, Tnani H, Puigdomènech P, Vicient CM
Meetup Presentations
Short courses Attended
Introduction to robotics
University of Coimbra
Coimbra, Portugal
2019
Bioinformatics and Genome analysis
Institut Pasteur de Tunis
Tunis, Tunisia
2017
References
Dr. Endang Septiningsih
Department of Soil and Crop Sciences, Texas A&M University
United States
N/A
- Email: eseptiningsih@tamu.edu